Aloysius J. Klingelhutz, Ph.D.
Ph.D., University of Wisconsin-Madison (Genetics), 1991 |
Associate Professor of Microbiology Campus address: 2202 MERF Mailing address: 375 Newton Rd. 2202 Medical Education and Biomedical Research Facility Iowa City, IA 52242 Phone: 319-335-7788 Email: |
Genetic factors involved in aging and cancer
In the broadest sense, my goal is to understand the biology and genetics of human cancer and aging. One of my primary interests is in how epithelial cells become immortal and subsequently malignant after infection with human papillomavirus (HPV). We are also studying how telomere loss and other factors are involved in induction of cellular senescence and aging. Specific areas of research are the following: 1) Examining the regulation of cellular genes by HPV E6 and E7 during the process of infection and transformation; 2) Determining the role of specific genomic alterations in the development of head and neck cancers; 3) Determining how the cell cycle inhibitor, p16INK4a, affects cell migration; 4) Characterizing how p16INK4a is regulated during telomere-independent senescence of human epithelial cells; 5) Defining the roles of telomere loss and telomerase dysfunction in cell aging and transformation; 6) Developing methods to extend cell lifespan by telomere restoration and regulation of cell cycle inhibitors It is hoped that these studies will increase our understanding of the processes that cause cancer and aging and that they will lead to better methods to prevent, diagnose, and treat human disease.
Additional information
UI Carver College of Medicine Interview
UI Interdisciplinary Graduate Program in Molecular Biology
UI Medical Scientist Training Program
Recent publications
Westin, E.R., E. Chavez, K.M. Lee, F.A. Gourronc, S. Riley, P.M. Lansdorp, F.D. Goldman, and A.J. Klingelhutz. Telomere restoration and extension of proliferative lifespan in dyskeratosis congenita fibroblasts. Aging Cell, In Press.
Berger, K.L., F. Barriga, M.J. Lace, L.P. Turek, G.J. Zamba, F.E. Domann, J.H. Lee, and A.J. Klingelhutz. 2006. Cervical keratinocytes containing stably replicating extrachromosomal HPV-16 are refractory to transformation by oncogenic H-Ras. Virology 356:68-78.
Darbro, B.W., K.M. Lee, N.K. Nguyen, F.E. Domann, and A.J. Klingelhutz. 2006. Methylation of the p16INK4a promoter region in telomerase immortalized human keratinocytes co-cultured with feeder cells. Oncogene, 25:7421-7433.
James, M.A., J.H. Lee, J.H., and A.J. Klingelhutz. 2006. HPV16-E6 associated hTERT promoter acetylation is E6AP dependent, increased in later passage cells, and enhanced by loss of p300. Int J Cancer 119:1878-1885.
James, M.A., J.H. Lee, and A.J. Klingelhutz. 2006. Human papillomavirus type 16 E6 activates NFkB, induces cIAP-2 expression, and protects against apoptosis in a PDZ binding motif-dependent manner. J Virol 80:5301-5307.
Darbro, B.W., G.B. Schneider, and A.J. Klingelhutz. 2005. Co-regulation of p16INK4a and migratory genes in culture conditions that lead to premature senescence in human keratinocytes. J Invest Derm 125:499-509.