Abstracts

21st Annual CBB Conference
"Current Topics in Industrial Biotechnology"

October 15-16, 2012
University of Iowa
Iowa Memorial Union, Iowa City, IA

Click here for Poster Preparation Instructions

21st Annual CBB Conference Poster Abstracts

EFFECTS OF Y209W ON THE HYDRIDE TRANSFER STEP IN THYMIDYLATE SYNTHASE
Thelma Abeysinghe, Zhen Wang and Amnon Kohen*
Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
PRODUCTION OF METHYLXANTHINES BY METABOLICALLY ENGINEERED E. Coli
Khalid H. R. Al-Gharrawi¹, Ryan M. Summers², Sridhar R. Gopishetty² and Mani Subramanian^{1, 2*}
¹Department of Chemical and Biochemical Engineering, College of Engineering, The University of Iowa, Iowa City, IA 52242
²Center for Biocatalysis and Bioprocessing, The University of Iowa Research Park, Coralville, IA 52241
DETERMINING PROTEIN BINDING SELECTIVITY OF N-ARYLACYL O-SULFONATED AMINOGLYCOSIDE DERIVATIVES
Ioana Craciun, Amanda M. Fenner and Robert J. Kerns*
Division of Medicinal and Natural Products Chemistry, College of Pharmacy, The University of Iowa, Iowa City, IA 52242
SYNTHESIS OF A MEMBRANE DISRUPTIVE GLYCOPROTEIN FOR GENE THERAPY
Samuel T. Crowley, Jianfeng Jin and Kevin G. Rice*
Division of Medicinal and Natural Products Chemistry, College of Pharmacy, The University of Iowa, Iowa City, IA 52242
DESIGN OF MULTIFUNCTIONAL RESURRECTING AGENTS FOR AGED AChE-OP ADDUCTS
Jacob D. Frueh and Daniel M. Quinn*
Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA, 52242
SOLVENT-ENHANCED BIOTRANSFORMATIONS OF CHEMICALS BY BEAUVERIA BASSIANA AS BIOCATALYST
Richard Gonzalez and Tonya L. Peeples*
Department of Chemical and Biochemical Engineering, College of Engineering, The University of Iowa, Iowa City IA 52242
MUTANT FORMATE DEHYDROGENASE STUDIES REVEAL ALTERED ACTIVE SITE DYNAMICS
Qi Guo, Christopher Cheatum*and Amnon Kohen*
Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
DETECTING ATRAZINE DEGRADATION IN PSEUDOMONS SP. STRAIN ADP BIOFILM WITH IN-SITU REVERSE TRANSCRIPTION AND RAMAN SPECTROSCOPY
Victoria A. Henry, Julie L. Jessop* and Tonya L. Peeples*
Department of Chemical and Biochemical Engineering, College of Engineering, The University of Iowa, Iowa City, IA 52242
EFFECTS OF THE MUTATION OF Arg166 IN THE HYDRIDE AND PROTON TRANSFERS IN THYMIDYLATE SYNTHASE
Zahidul Islam, Zhen Wang and Amnon Kohen*
Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
TOWARD DETERMINING THE FUNCTION OF RLPA, A CELL DIVISION PROTEIN FROM PSEUDOMONAS AERUGINOSA
Matthew A. Jorgenson^1,2 David L. Popham³ and David S. Weiss^1,2*
¹Interdisciplinary Program in Genetics, The University of Iowa, Iowa City, IA 52242
²Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, The University of Iowa, Iowa City, IA 52242
³Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061
SMALL MOLECULE OXIDATIVE METABOLISM INHIBITORS FOR METASTATIC MELANOMA THERAPY
Kyle C. Kloepping, Mitchell C. Coleman, Brett A. Wagner, James A. Jacobus, Kranti A. Mapuskar, Garry R. Buettner, Douglas R. Spitz and Michael K. Schultz*
Department of Radiation Oncology (Free Radical Radiation Biology Program), Roy J. and Lucille A. Carver College of Medicine, The University of Iowa, Iowa City, IA 52242
DESIGN AND SYNTHESIS OF NOVEL N-1 FLUOROQUINOLONES
Chaitanya A. Kulkarni, Tyrell R. Towle and Robert J. Kerns*
Division of Medicinal and Natural Products Chemistry, College of Pharmacy, The University of Iowa, Iowa City, IA 52242
A SYNTHETIC APPROACH TO MEROTERPENOID PHOMOARCHERIN C
Tara A. Ladlie, Nolan R. Mente and David F. Wiemer*
Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
CO-CRYSTALS OF COMPLEMENTARY BIFUNCTIONAL TEMPLATES
Rebecca C. Laird, Nam P. Nguyen and Leonard R. MacGillivray*
Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
MICROBIAL POLYCHLORINATED BIPHENYL DEGRADATION IN SWITCHGRASS PLANTED RHIZOSPHERE
Yi Liang, Richard Meggo, Jerald L. Schnoor and Timothy E. Mattes*
Department of Civil and Environmental Engineering, College of Engineering, The University of Iowa, Iowa City, IA 52242
SPECIFICITY, STRUCTURE, DYNAMICS AND INHIBITION OF TIAM1 PDZ DOMAIN/SYNDECAN1 COMPLEX
Xu Liu¹, Tyson R. Shepherd¹, Yu Chen² and Ernesto J. Fuentes¹*
¹Department of Biochemistry, Roy J. and Lucille A. Carver College of Medicine, The University of Iowa, Iowa City, IA 52242
²Department of Molecular Medicine, University of South Florida, Tampa, FL 33612
SYNTHESIS AND EVALUATION OF HUMAN ACETYLCHOLINESTERASE INHIBITORS
Alexander Lodge, Joseph Topczewski and Daniel Quinn*
Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
TARGETING THE G?o: RGS17 PROTEIN: PROTEIN INTERACTION FOR THE TREATMENT OF LUNG AND PROSTATE CANCERS
Duncan I. Mackie¹ and David L. Roman^1,2*
¹Division of Medicinal and Natural Products Chemistry, College of Pharmacy, The University of Iowa, Iowa City, IA 52242
²Holden Comprehensive Cancer Center, The University of Iowa Hospitals and Clinic, Iowa City, IA 52242
HYPOXIA INDUCIBLE FACTOR DEPENDENT OXYGEN SENSING
Steve Mather, James Latanski, Sulay Patel and Mishtu Dey*
Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
TOWARDS BETTER ANTIBIOTICS: STUDIES OF ALTERNATIVE THYMIDYLATE SYNTHASE
Tatiana V. Mishanina, Eric M. Koehn, John A. Conrad, Bruce A. Palfey, Scott A. Lesley and Amnon Kohen*
Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
DEVELOPMENT OF A RAPID COLORIMETRIC ASSAY FOR DETECTION OF CAFFEINE IN BEVERAGES AND BODY FLUIDS USING CAFFEINE DEHYDROGENASE FROM PSEUDOMONAS sp. CBB1
Sujit K. Mohanty¹, Chi-Li Yu^1,2, Sridhar Gopishetty² and Mani Subramanian^1,2*
¹Department of Chemical and Biochemical Engineering, College of Engineering, The University of Iowa, Iowa City, IA 52242
²Center for Biocatalysis and Bioprocessing, The University of Iowa Research Park, Coralville, IA 52241
SCREENING BEAUVARIA BASSIANA OXIDATIVE ENZYMES
Felipe Nicolau-Manterola¹, Richard Gonzalez¹, Tonya L. Peeples¹* and Horacio F. Olivo²*
¹Department of Chemical and Biochemical Engineering, College of Engineering, The University of Iowa, Iowa City, IA 52242
²Division of Medicinal and Natural Products Chemistry College of Pharmacy, The University of Iowa, Iowa City, IA 52242
COMPARISON OF CONFOCAL FLUORESCENT MICROSCOPY AND DIRECT ENUMERATION AS A MEANS OF QUANTIFYING DEACTIVATION OF BIOFILM
Ann O’Toole and Eric Nuxoll*
Department of Chemical and Biochemical Engineering, College of Engineering, The University of Iowa, Iowa City, IA 52242
CLOSTRIDIUM DIFFICILE VIRULENCE REQUIRES NOVEL CELL SHAPE FACTORS
Eric M. Ransom, David S. Weiss* and Craig D. Ellermeier*
Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, The University of Iowa, Iowa City, IA 52242
GENERATING A UNIFORM TISSUE PHANTOM WITH HYDROPHOBIC NANOPOROUS SILICA PARTICLES: TOWARD THE QUANTIFICATION OF PARTICLE DISPERSION
Cicily J. Ronhovde and M. Lei Geng*
Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
STAPHYLOCOCCUS AUREUS HYALURONATE LYASE, A POTENT VIRULENCE FACTOR
Carolyn B. Rosenthal¹, Crystal L. Jones², Jay K. Moon², Chad C. Black², Daniel V. Zurawski² and Alexander R. Horswill¹*
¹Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, The University of Iowa, Iowa City, IA 52242
²Walter Reed Army Institute of Research (WRAIR), Department of Wound Infections, Silver Spring, MD 20901
TWO COMPLEMENTARY APPROACHES ILLUMINATE THE HYDRIDE TRANSFER CATALYZED BY DIHYDROFOLATE REDUCTASE
Danial Roston¹, Christopher M. Cheatum¹*, Dan T. Major² and Amnon Kohen¹*
¹Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
²Department of Chemistry and the Lise Meitner-Minerva Center of Computational Quantum Chemistry, Bar-Ilan University, Ramat-Gan 52900, Israel
ENZYMOLOGY OF DIFFERENT DIMETHYLSULFONIOPROPIONATE BIODEGRADATION PATHWAYS
Nicholas J. Schnicker, Alexander Crider and Mishtu Dey*
Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
STUDIES TOWARDS SYNTHESIS OF TRUNCATED SUPERSTOLIDE A
Aashay K. Shah, Lei Chen and Zhendong Jin*
Division of Medicinal and Natural Products Chemistry, College of Pharmacy, The University of Iowa, Iowa City, IA 52242
GLOBAL NETWORKS IN ENZYMATIC CATALYSIS: STUDIES OF DIHYDROFOLATE REDUCTASE
Priyanka Singh and Amnon Kohen*
Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
ENDOXIFEN AND OTHER METABOLITES OF TAMOXIFEN INHIBIT HUMAN HYDROXYSTEROID SULFOTRANSFERASE hSULT2A1
Edwin J. Squirewell¹, Xiaoyan Qin^1,2 and Michael W. Duffel^1,2*
¹Division of Medicinal and Natural Products Chemistry, College of Pharmacy, The University of Iowa, Iowa City, IA 52242
²Interdisciplinary Graduate Program in Human Toxicology, The University of Iowa, Iowa City, IA 52242
GAPDH IS A TARGET OF THE ENDOGENOUS 3,4-DIHYDROXYPHENYLACETALDEHYDE: ENZYME INHIBITION AND COVALENT MODIFICATION
Brigitte C. Vanle, Virginia Florang and Jonathan Doorn*
Division of Medicinal and Natural Products Chemistry, College of Pharmacy, The University of Iowa, Iowa City, IA 52242
ACCOUNTING FOR INTERSTITIAL WATERS IN INHIBITOR DESIGN: EVIDENCE OF ANACTIVE ROLE OF WATER IN GLUTAMATE RACEMASE
Katie Whalen^1,2, Joseph Reese¹, Wesley Lay², Eric Ahn¹, Kenneth Tussey² and Ashley Spies²*
¹Department of Biochemistry, University of Illinois, Urbana, IL 61801
²Division of Medicinal and Natural Products Chemistry, College of Pharmacy, The University of Iowa, Iowa City, IA 52242
COMPUTATIONAL STUDIES SUGGEST A NEW REACTION INTERMEDIATE FOR THYMIDYLATE SYNTHASE
Zhen Wang¹, Silvia Ferrer², Vicent Moliner²* and Amnon Kohen¹*
¹Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
²Departament de Química Física i Analítica, Universitat Jaume I, 12071 Castellón, Spain
NOVEL FLUOROQUINOLONE ANALOGS WITH ANTIMUTANT ACTIVITY
Benjamin H. Williamson, Heidi A. Schwanz and Robert J. Kerns*
Division of Medicinal and Natural Products Chemistry, College of Pharmacy, The University of Iowa, Iowa City, IA 52242
APPROACHES TO SYNTHESIS OF THE STELLETTINS
Veronica S. Wills and David F. Wiemer*
Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
METABOLIC ENGINEERING OF E. COLI FOR PRODUCTION OF HIGH-VALUE METHYLXANTHINES FROM CAFFEINE, THEOPHYLLINE, AND THEOBROMINE
Ryan M. Summers², Sridhar Gopishetty² and Mani Subramanian^1,2*
¹Department of Chemical and Biochemical Engineering, College of Engineering, The University of Iowa, Iowa City, IA 52242
²Center for Biocatalysis and Bioprocessing, The University of Iowa Research Park, Coralville, IA 52241
DESIGN OF BIO-COMPATIBLE METHYL TRANSFER AGENTS FOR THE POTENTIAL RESURRECTION OF AGED AChE-OP ADDUCTS
Joseph J. Topczewski and Daniel M. Quinn*
Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
STRUCTURE DETERMINATION AND PEPTIDOGLYCAN BINDING ASSAYS OF SPOR DOMAIN FROM THE Esherichia COLI CELL DIVISION PROTEIN DamX
Atsushi Yahashiri¹, Kyle B. Williams¹, C. Andrew Fowler² and David S. Weiss¹*
¹Department of Microbiology, Roy J. and Lucille A. Carver College of Medicine, The University of Iowa, Iowa City, IA 52242
²NMR Core Facility, Roy J. and Lucille A. Carver College of Medicine, The University of Iowa, Iowa City, IA 52242
DYNAMIC RESOLUTION OF CHIRAL 2-HYDROXY ACIDS BY SPINACH GLYCOLATE OXIDASE AND SUBSEQUENT PURIFICATION OF THE (R)-ENANTIOMERS USING SIMULATED MOVING BED CHROMATOGRAPHY
Shuvendu Das², Anthony Grabski³, Bruce Thalley³, Alla Zilberman³, Robert Mierendorf³ and Mani Subramanian^1,2*
¹Department of Chemical and Biochemical Engineering, College of Engineering, The University of Iowa, Iowa City, IA 52242
²Center for Biocatalysis and Bioprocessing, The University of Iowa Research Park, Coralville, IA 52241
³Semba Biosciences, Inc., 505 South Rosa Road, Madison, WI 53719
MODULAR AND EXPANDABLE DETECTION PLATFORM FOR CURRENT AND POTENTIAL FOOD TOXINS AND ADULTERANTS: IDENTIFICATION AND ISOLATION OF RICININE NITRILASE FROM MICROBIAL SCREENING AND SOIL ISOLATES
Chi Li Yu^1,2, Bhavita R. Bhatt¹, Tony Dodge³, Michael Sadowsky³, Lawrence P. Wackett³ and Mani Subramanian^1,2*
¹Department of Chemical and Biochemical Engineering, College of Engineering, The University of Iowa, Iowa City, IA 52242
²Center for Biocatalysis and Bioprocessing, The University of Iowa Research Park, Coralville, IA 52241
³Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, St. Paul, MN 55108
SCALABLE HIGH THROUGHPUT SCREENING AND ITS APPLICATIONS
Meng Wu, Ph.D.
High Throughput Screening Facility, College of Pharmacy, The University of Iowa, Iowa City, IA 52242
CONTINUOUS, REAL-TIME MONITORS FOR PICHIA PASTORIS BIOREACTORS
Elizabeth R. Gibson¹, Ed Koerperick¹, Kaylee Lanz¹, Jonathon T. Olesberg^1,2, Gary W. Small^1,2 and Mark A. Arnold^1,2
¹ASL Analytical, Inc., Coralville, IA 52241
²Optical Science and Technology Center and Department of Chemistry, College of Liberal Arts and Sciences, The University of Iowa, Iowa City, IA 52242
RoboLector® FAMILY-FROM AUTOMATED MEDIA PREPARATION TO FED-BATCH FERMENATION IN SHAKEN MICROBIOREACTORS
Liz Dierickx, Pamela Wenk, Johannes Hemmerich and Frank Kensy
m2p-labs, Inc. (NY, USA & Baesweiler, Germany
REGULATION OF CALCINEURIN BY DOMAIN-SPECIFIC INTERACTIONS WITH CALMODULIN
Sean Klein, Brett C. Waite, Susan E. O’Donnell, Jesse B. Yoder, and Madeline A. Shea*
Department of Biochemistry, Roy J. and Lucille A. Carver College of Medicine, The University of Iowa, Iowa City, IA 52242
ROLE OF HOMOLOG CuZnSOD IN BACULOVIRUS INFECTION IN INSECT CELLS
Bhakti Bapat and David Murhammer*
Department of Chemical and Biochemical Engineering, College of Engineering, The University of Iowa, Iowa City, IA 42242

POSTER PREPARATION

(1)	The poster board has a 44" H x 68" W area for mounting the posters.
(2)	Lettering and illustrations on the poster pages must be large enough to be read by attendees from a distance of 3 feet or more.
(3)	Each poster must have a title, authors and affiliation section at the beginning. Material should be displayed in logical sequence (e.g., introduction, objectives, results, conclusion) and each sheet should be numbered.
(4)	Authors are responsible for mounting their own poster material on poster boards which will be provided at the conference. The Center will provide a reasonable supply of clear push pins. Posters should be removed immediately following the poster session and before dinner, since the poster boards are scheduled to be removed for use elsewhere.