GLP Facilities

The 16,000 ft² CBB GLP laboratory/office offers state-of-the-art fermentation/bioprocessing equipment and an experienced professional full-time staff with expertise in process development. For example, our contract culturing capabilities include:

	Large-capacity multi-tier shakers, in environmentally controlled rooms,
	Eight two-liter fermentors, used for optimization,
	A total of 23 fermentors to 1,000-liter working capacity,
	A liquid-oxygen supply system for high-cell-density culture at 100- and 1,000-liter scale, and
	A tandem 25-ton glycol cooling system.

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As an example of capabilities, CBB has performed 100-liter fermentations with in-situ microfiltration, as well as fermentations with simultaneous product removal by adsorption. CBB has performed many unique chromatographic separations to recover valuable products. State-of-the-art downstream capabilities match the scale of the fermentors. A summary of holdings is outlined below, and tabulated details follow the summary.

	Fundamentals include a large medium preparation room, sterilizers, and washer. Multi-tier, high-capacity shakers are housed in 29°C and 37°C incubation rooms. The laboratory also contains two 4°C cold rooms, as well as supporting infrastructure, including an explosion-proof solvent-extraction room with walk-in hood, laboratory benches, etc.
	Principal fermentors are listed in Table 1. Most fermentors have PC-based, on-line measurement and control capabilities. All have systems capable of handling explosive and flammable gases as well as aseptic sample collection and inoculation. Most fermentors have on-line, off-gas analysis capability. In addition, a clean-in-place system is available to service large fermentors. A liquid-oxygen system is available to provide high rates to 100-and 1,000-liter high-cell-density fermentations. In addition, a high-capacity glycol cooling system can rapidly chill fermentors and holding tanks.
	Analytical services are partially listed in Table 2. The liquid chromatography unit is both analytical and preparative scale and has processed up to 36 L/h.
	CBB is fully prepared for downstream, pilot-scale operations in fermentation, harvesting, and bioprocessing. For example, the filtration system has recovered low-molecular weight materials while processing more than 200 L/h. Table 3 provides a partial listing of downstream processing capabilities.
	CBB has several protein purification systems. For purifications under GLP status, CBB has an Akta Explorer Chromatography system and an Akta Pilot chromatography system. . . . more
	Detailed information regarding the GLP Equipment may be found here (pdf file)

Questions about CBB contract fermentation should be directed to Marvin Scher.