The Xenogen IVIS-200 System is a high-sensitivity, low noise, in vivo imaging technology platform that allows non-invasive visualization and tracking of cellular and genetic activity within a living organism, in real time. It’s capable of imaging bioluminescence and fluorescence. IVIS-200 provides Living Image® software for image display and analysis.
There are fluorescence filter sets available on the system are as follows:
Filter Set |
Label |
Excitation |
Emission Passband |
Dyes &Passband |
1 |
GFP |
445-490 |
515-575 |
GFP,EGFP, FITC |
2 |
DsRed |
500-550 |
575-650 |
DsRed2-1, PKH26,CellTrackerOrange |
3 |
Cy5.5 |
615-665 |
695-770 |
Cy5.5, Alexa Fluor® 660, Alexa Fluor®680 |
4 |
ICG |
710-760 |
810-875 |
Indocyanine gree (ICG) |
Specifications:
1. CCD Cameras
CCD Camera |
Specification |
Sensor |
Back Illuminated |
CCD Format |
2048 x 2048 pixels |
CCD Size |
26 x 26 mm |
Effective CCD Format |
1920 x 1920 pixels |
Pixel Size |
13.5 x 13.5u,m |
Quantum Efficiency |
~857o 400-700nm |
Readout Noise |
<5 electrons RMS |
Dark Current |
<100 electrons/s/cm2 (-105°C) |
Minimum Detectable Luminance |
<70 photons/s/sr/cm2 |
CCD Temperature |
Nominal -105°C |
2. Optics
Lens f/stop |
f/1 -f/8 |
Field of Views (FOV) |
3.9, 6.5, 13, 19.5 and 26cm |
Resolution |
>60u,m (FOV=3.9, f/1 ) |
Scanning Laser |
<1 mW at 532 nm |
3. Fluorescence Imaging Components
Excitation Filters |
6 positions, 25 mm diameter |
Emission Filters |
22 positions, 60 mm diameter |
Background (autofluorescence and leakage) |
1 part in 107 typical |
Lamp |
150 W Quartz halogen 21 V 3250° Kelvin |
