"Atomic Force Microscopy: Applications for Life Science Research"
Applications of Atomic Force Microscopy (AFM) are ubiquitous in all bioscience areas that require surface characterization of molecules (nucleic acids, lipids, proteins…), cells (live mammalian cell cultures, bacteria…), tissues (bone, cartilage, dentin, cornea…), and biomaterials. Sample preparation is minimal and doesn't require fixation, coating, dehydration steps that may be necessary for other analysis techniques. As a result, the AFM allows for high resolution, three-dimensional imaging of samples in conditions close to their native state.
Another important benefit of AFM is that quantitative force measurements on the picoNewton scale can be conducted on your samples. These measurements include protein unfolding, adhesion forces and cell elasticity.
During this presentation we will present a brief overview of the principles of AFM and then discuss more extensively the applications in the Life Sciences.
"Basic Principles and Technology of Atomic Force Microscopy"
Atomic Force Microscopy is a technique of choice for non-destructive, high resolution imaging in many applications areas including biology, material science, electrochemistry, polymer science, data storage, magnetism, and semiconductors. In addition to surface topography, it also allows picoNewton resolution force measurements for single molecule mechanical studies (protein unfolding, adhesion, etc.).
In order to maximize the performance of an AFM, it is important to understand how an AFM works and how each component of the microscope plays a role in the optimization of these results. This talk will begin by explaining the basic principles of AFM. Then we will describe the design of the AFM, both as a stand-alone instrument as well as an integrated system with an inverted optical microscope. Several examples will be presented including data acquired using an AFM/optical microscopy system, such as combined AFM with epifluorescence, confocal, TIRF and optical phase contrast.Instructor: Sophia Hohlbauch
Host: Jean Ross
Date: September 28th
Time & Location: 10am-11am Lecture MacEwan
mmmmmmmmmmmi1pm - 2pm Demonstration 87 CMRFCurrent Enrollment: