The identification of elemental composition in biological samples by means of electron-specimen interactions has always been problematic due to a poor sample resistance to a high energy electron beam. High energy electrons in Energy Dispersive X-Ray (EDX) analysis can damage and/or destroy the sample in the matters of seconds. Additionally, bulk information obtained offers very low signal-to-noise ratio in terms of element of interest to carbon.
The new alternative is viable via X-ray Photoelectron Spectroscopical (XPS) analysis, where sample is probed only at the very surface. The information obtained can be propagated to get the better understanding of the elemental composition of the sample. This is especially true when probing thin sections of the biological material.
The elemental quantification of problematic biological samples via XPS analysis will be offered in the IMS 2006 meeting is a novel approach to better understand elemental composition. Those interested should contact Jonas Baltrusaitis at
jonas-baltrusaitis@uiowa.edu or 5-8142 several days in advance to discuss the samples. The useful reference is:
Kiss, Peter J. et al., Inactivation of NADPH oxidase organizer 1 Results in Severe Imbalance. Current Biology (2006), 16(2), 208-213.
Hong, Lian; Simon, John D. Physical and chemical characterization of iris and choroid melanosomes isolated from newborn and mature cows. Photochemistry and Photobiology (2005), 81(May/June), 517-523.
If interested in this workshop please register with the date you would like to participate and email Jonas with your available time.
Instructor: Jonas Baltrusaitis
Host: Jonas Baltrusaitis
Date & Time:
September 28th: Available all day
September 29th: Available all day
Location: 88 EMRB
Current Enrollment:
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