Free Radical Journal Club

The University of Iowa

Spring 2000

12:00-1:00, Wednesdays

Room B180, Med Labs

Contact: garry-buettner@uiowa.edu Tel 319/335-6749

 

January 19, 2000
Presenter: Dr. Mike McCormick
Paper: Rota C et al. (1999) Phenoxyl free radical formation during the oxidation of the fluorescent dye 2 ',7 '-dichlorofluorescein by horseradish peroxidase - Possible consequences for oxidative stress measurements. J Biol Chem 274: 28161-28168.

January 26, 2000
Presenter: Dr. Dave Hall
Paper: Estevez AG et al. (1999) Induction of nitric oxide ¿ dependent apoptosis in motor neurons by zinc-deficient superoxide dismutase. Science 286: 2498-2500.

February 02, 2000
Presenter: Jeff Kirsch
Paper: Nishiyama A et al. (1999) Identification of thioredoxin-binding Protein-2/vitamin D3 up-regulated Protein 1 as a negative regulator of thioredoxin function and expression. J Biol Chem 274: 21645-21650.

February 09, 2000
Presenter: Brett Wagner
Paper: Vissers MCM et al. (1999) Hypochlorous acid causes caspase activation and apoptosis or growth arrest in human endothelial cells. Biochem J. 344: 443-449.

February 16, 2000
Presenter: Dr. Freya Schafer
Paper: Kirlin WG et al. (1999) Glutathione redox potential in response to differentiation and enzyme inducers. Free Radic Biol Med. 27(11-12):1208-1218.

February 23, 2000
Presenter: Shawn Flanagan
Paper: Ciriolo MR et al. (2000) Cu,Zn-superoxide dismutase-dependent apoptosis induced by nitric oxide in neuronal cells. J Biol Chem 275: 5065-5072.

March 01, 2000
Presenter: Dr. Larry Oberley
Paper: Schmidt PJ et al. (1999) Multiple protein domains contribute to the action of the copper chaperone for superoxide dismutase. J Biol Chem 274: 23719-23725. Schmidt PJ et al. (1999) A gain of superoxide dismutase (SOD) activity obtained with CCS, the copper metallochaperone for SOD1. J Biol Chem 274: 36952-36956.

March 08, 2000
Presenter: Michael A. Mackey PhD
Dr. Mackey is being recruited for both the College of Engineering and the Department of Radiology. He will give an informal presentation of the following abstract during his time scheduled with us.
"The induction of mitotic catastrophe following stress exposure of p53-deficient cells."

March 22, 2000
Presenter: Dr. Doug Spitz
Paper: Diamond DA et al. (1999) Redox factor-1 (Ref-1) mediates the activation of AP-1 in HeLa and NIH 3T3 cells in response to heat shock. J Biol Chem 274: 16959-16964. Curry HA et al. (1999) Heat shock inhibits radiation-induced activation of NF-kB via inhibition of I-kB kinase. J Biol Chem 274: 23061-23067.

March 29, 2000
Presenter: Jianfang Hu
Paper: Monteiro HP et al. (2000) Nitric oxide stimulates tyrosine phosphorylation of focal adhesion kinase, SRC kinase, and mitogen-activated protein kinases in murine fibroblasts. Free Radic Biol Med 28: 174-182.

April 05, 2000
Presenter: Dr. Garry Buettner
Paper: Lass A, Sohal RS. (2000) Effect of coenzyme Q10 and a -tocopherol content of mitochondria on the production of superoxide anion radicals. FASEB 14: 87-94.

April 12, 2000
Presenter: Min Wang
Paper: Zaman K et al. (1999) Protection from oxidative stress-induced apoptosis in cortical neuronal cultures by iron chelators is associated with enhanced DNA binding of Hypoxia-Inducible Factor-1 and ATF-1/CREB and increased expression of glycolytic enzymes, p21waf1/cip1, and erythropoietin. J Neurosci 19: 9821-9830.

April 19, 2000
Presenter: Chris Darby
Paper: Rieber M, Rieber MS. (1999) Tumor Suppression without Differentiation or Apoptosis by Antisense cyclin D1 Gene Transfer in K1735 Melanoma Involves Induction of p53, p21WAF1 and Superoxide Dismutases. Cell Death and Differentiation 6: 1209-1215.

April 26, 2000
Presenter: Eric Kelley
Paper: Rubbo H et al. (2000) Nitric oxide reaction with lipid peroxyl radicals spares alpha-tocopherol during lipid peroxidation. J Biol Chem 275: 10812-10818.

May 03, 2000
Presenter: Jeanie Kirk
Paper: Caniggia I. Mostachfi H. Winter J. Gassmann M. Lye SJ. Kuliszewski M. Post M. Hypoxia-inducible factor-1 mediates the biological effects of oxygen on human trophoblast differentiation through TGFbeta(3). Journal of Clinical Investigation. 105(5): 577-87, 2000.

May 10, 2000
Presenter: Sujatha Venkataraman
Paper: Godber BLJ et al. (2000) Reduction of nitrite to nitric oxide catalyzed by xanthine oxidoreductase. J Biol Chem 275: 7757-7763.

Papers that will not be discussed, but provide additional background are:
Rota C et al. (1999) Evidence for free radical formation during the oxidation of 2 '-7'-dichlorofluorescin to the fluorescent dye 2 '-7 '-dichlorofluorescein by horseradish peroxidase: Possible implications for oxidative stress measurements Free Radic Biol Med 27: 873-881.
Hempel SL, Buettner GR, O'Malley YQ, et al. (1999) Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2 ',7 ' dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med 27: 146-159