| Gene Transfer Vector Core In-Stock Vectors
Stec DE, Davisson RL, Haskell RE, Davidson BL, Sigmund CD. Efficient liver-specific deletion of a floxed human angiotensinogen transgene by adenoviral delivery of CRE-Recombinase in vivo. J Biol Chem 274(30):21285-21290, 1999.
Berman SD, Calo E, Landman AS, Danielian PS, Miller ES, West JC, Fonhoue BD, Caron A, Bronson R, Bouxsein ML, Mukherjee S, Lees JA. Metastatic osteosarcoma induced by inactivation of Rb and p53 in the osteoblast lineage. Proc Natl Acad Sci. Aug 19;105(33):11851-6, 2008
Woolfenden S, Zhu H, Charest A, A Cre/LoxP conditional luciferase reporter transgenic mouse for bioluminescence monitoring of tumorigenesis. Genesis. Oct;47(10):659-66, 2009.
Cre Ad5 GFAP, nestin, and hSynapsin shuttle plasmids are courtesy of Dr. Alain Charest's Laboratory, Tufts Medical Center.
Sinnayah P, Lindley TE, Staber PD, Davidson BL, Cassell MD, Davisson RL. Targeted viral delivery of Cre recombinase induces conditional gene deletion in cardiovascular circuits of the mouse brain. Physiol Genomics. 2004 Jun 17;18(1):25-32.
AAV2 Cre injection (1.1E14 vg/ml) dorsal to the substantia nigra (SN) and sacrificed for immunohistochemical analysis four weeks later. (A) Immunostaining for Cre. The upper panel demonstrates immunoperoxidase staining for Cre in the SN pars compacta (SNpc) on the injected side of the brain. The lower panels demonstrate by immunofluorescence double-labeling that many tyrosine hydroxylase (TH) - positive neurons express Cre. (B) Immunohistochemical analysis of b-galactosidase. Immunoperoxidase labeling is observed in the SNpc on the injected side of the brain, demonstrating that Cre-mediated recombination has occurred. (C) Immunoperoxidase staining of TH-positive neurons at four weeks post-injection of AAV2 demonstrates that there is no toxicity for SN dopamine neurons, confirmed by stereologic counts (N = 4; Control: 6675 ± 203; Injection Side: 6762 ± 378, p = 0.9, NS).
(Images courtesy of the Burke Laboratory, Department of Neurology, Columbia University)
(Cre AAV shuttle plasmid courtesy of Dr. Robert Burke's Laboratory,
Department of Neurology, Columbia University)