Publications of David Price

2000-2009

 

Price, D.H. (2009) Guest Editor's Introduction.  Methods, 48:321-2.

 

Cheng, B and Price, D.H. (2009) Isolation and functional analysis of RNA polymerase II elongation complexes.  Methods 48:346-52.    

 

Cheng, B and Price, D.H. (2008) Analysis of factor interactions with RNA polymerase II elongation complexes using a new electrophoretic mobility shift assay.  Nuc. Acids Res. doi:10.1016/j.ymeth.2009.02.026 .

 

Gurumurthy, M., Tan, G., Ng, R., Zeiger, L., Lau, J., Lee, J., Dey, A., Philp, R., Li, Q., Lim, T., Price, D.H., Lane, P.l, and Chao, S-H. (2008) Nucleophosmin interacts with HEXIM1 and regulates RNA polymerase II transcription.  J. Mol. Biol. 378:302-317.

 

Krueger, B.K., Jeronimo, C., Roy, B.B., Bouchard, A., Barrandon,C., Byers, S.A., Searcey, C.E., Cooper, J.J., Bensaude, O., Cohen, E.A., Coulombe, B, and Price, D.H. (2008) LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated.  Nucl. Acids Res. 36:2219-29.

 

Price, D.H. Poised polymerases: on your mark…get set…go! (2008) (minireview). Mol. Cell 30:7-10.

 

Biglione, S., Byers, S.A., Price, J.P., Nguyen, V.T., Bensaude, O., Price, D.H., and Maury, W.J. (2007) Inhibition of HIV-1 replication by P-TEFb inhibitors DRB, Seliciclib and Flavopiridol correlates with release of free P-TEFb from the large form of the complex.  Retrovirology 4:47.

 

Jeronimo, C., Forget, D., Bouchard, A., Li, Q., Chua, G., Poitras, C., Thérien, C., Bergeron, D., Bourassa, S., Greenblatt, J., Chabot, B., Poirier, G.G., Hughes, T.R., Blanchette, M., Price, D.H. and Coulombe, B. (2007) Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme.  Mol. Cell 27: 262-74

 

Sedore, S.C., Byers, S.A., Biglione, S., Maury, W.J., and Price, D.H. (2007) Manipulation of P-TEFb control machinery by HIV: Recruitment of P-TEFb from the large form by Tat and binding of HEXIM1 to TAR.  Nuc. Acids. Res. 35:4347-58. 

 

Cheng, B. and Price, D.H. (2007) Properties of RNA polymerase II elongation complexes before and after the P-TEFb-mediated transition into productive elongation.  J. Biol. Chem. 282:21901-12.

 

Li, Q., Cooper, J.J., Altwerger, G.H., Feldkamp, M.D., Shea, M.A., and Price, D.H. (2007) HEXIM1 is a promiscuous double-stranded RNA binding protein and interacts with RNAs in addition to 7SK in cultured cells.  Nuc. Acids. Res. 25:2503-2512.

 

Peterlin, B.M, Price, D.H (2006) Controlling the elongation phase of transcription with P-TEFb.  Mol. Cell 23:297-305.

 

Barboric, M., Kohoutek, J., Price, J.P., Blazek, D., Price, D.H., Peterlin, B.M. (2005) Interplay between 7SK snRNA and oppositely charged regions in HEXIM1 direct the inhibition of P-TEFb.  EMBO J. 24:4291-303.

 

Palangat M, Renner D.B., Price D.H., Landick R. (2005) A negative elongation factor for human RNA polymerase II inhibits the anti-arrest transcript-cleavage factor TFIIS.  Proc. Natl. Acad. Sci. USA 102:15036-41. 

 

Adamson, T.E., Shutt, D.C., and Price, D.H. (2005) Functional coupling of cleavage and polyadenylation with transcription of mRNA.  J. Biol. Chem. 280:32262-71. 

 

Byers, S.A., Schafer, B., Sappel, D.S., Brown, J., and Price, D.H. (2005) The anti-proliferative agent, MLN944, preferentially inhibits transcription.  Molecular Cancer Therapeutics 3:1260-7.

 

Li Q, Price JP, Byers SA, Cheng D, Peng J, Price DH. (2005) Analysis of the large inactive P-TEFb complex indicates that it contains one 7SK molecule, a dimer of HEXIM1 or HEXIM2, and two P-TEFb molecules containing Cdk9 phosphorylated at threonine 186.  J. Biol. Chem. 280:28819-26. 

 

Byers, S.A., Price, J.P., Cooper, J.J., Li, Q, and Price, D.H. (2005) HEXIM2, a HEXIM1 related protein regulates P-TEFb through association with 7SK.  J. Biol. Chem. 280:16360-16367. 

 

Shore, S.M., Byers, S.A., Dent, P., and Price, D.H. (2005) Characterization of Cdk955 and differential regulation of two Cdk9 isoforms.  Gene 350:51-58. 

 

Jiang, Y. and Price, D.H. (2004) Rescue of the TTF2 knockdown phenotype with an siRNA-resistant replacement vector.  Cell Cycle 23:1151-1153.

 

Michels, A.A., Fraldi, A., Li, Q, Adamson, T.E., Bonnet, F, Nguyen, V.T., Sedore, S.C., Price J. P., Price, D.H., Lania, L., and Bensaude, O. (2004) Binding of the 7SK snRNA turns the HEXIM1 protein into a P-TEFb (Cdk9/CyclinT) inhibitor.  EMBO J. 23:2608-2619.

 

Jiang, Y. Liu, M. Spencer, C.A., and Price D.H. (2004) Involvement of Transcription Termination Factor 2 in mitotic repression of transcription elongation.  Mol. Cell 14:375-386. 

 

Adamson, T.E. and Price, D.H. (2003) Co-transcriptional processing of histone mRNAs.  Mol. Cell. Biol. 23:4046-4055. 

 

Shore, S.M., Byers, A.B., Maury, W., and Price, D.H. (2003) Identification of a novel isoform of Cdk9.  Gene 301:175-182. 

 

Moteki, S. and Price, D. H. (2002) Functional coupling of capping and transcription of mRNA.  Mol. Cell 10:599-609.

 

Renner, D. B., Yamaguchi, Y., Wada, T., Handa, H., and Price, D. H. (2001) A highly purified RNA polymerase II elongation control system.  J. Biol. Chem. 276:42601-9. 

 

Zhou, M., Nekhai, S., Bharucha, D. C., Kumar, A., Ge, H., Price, D. H., Egly, J. M., and Brady, J. N. (2001) TFIIH inhibits CDK9 phosphorylation during human iummunodeficiency virus Type 1 transcription.  J. Biol. Chem. 276:44633-44640.

 

Zhao, S-H., Price, D.H. (2001) Flavopiridol inactivates P-TEFb and blocks most RNA polymerase II transcription in vivo.  J. Biol. Chem. 276:31793-31799.

 

Chao, S-H., Greenleaf, A. L., Price, D.H. (2001) Juglone, an inhibitor of the peptidyl-prolyl isomerase Pin1, also directly blocks transcription.  Nucleic Acids Res. 29:767-773. 

 

Sune, C., Goldstrohm, A.C., Peng, J., Price, D.H., Garcia-Blanco, M.A. (2000) An in vitro transcription system that recapitulates equine infectious anemia virus tat-mediated inhibition of human immunodeficiency virus type 1 Tat activity demonstrates a role for positive transcription elongation factor b and associated proteins in the mechanism of Tat activation.  Virology, 274:356-366.

 

Chao, S-H., Fujinaga, K., Marion J.E., Taube, R., Sausville, E.A., Senderowicz, A.M., Peterlin, B.M., and Price, D.H. (2000) Flavopiridol inhibits P-TEFb and blocks HIV-1 replication.  J. Biol. Chem. Accelerated Publication 275:28345-28348.  

 

Zhou M, Halanski, M.A., Radonovich, M.F., Kashanchi, F., Peng, J., Price, D.H., and Brady, J.H. (2000) Tat modifies the activity of Cdk9 to phosphorylate serine 5 of the RNA polymerase II CTD during HIV-1 transcription.  Mol. Cell. Biol. 20:5077-5086. 

 

Lis, J.T., P. Mason, J. Peng, D.H. Price, and J. Werner. (2000) P-TEFb kinase recruitment and function at heat shock loci.  Genes & Dev. 14:792-803.

 

Price, D.H. (2000) P-TEFb, a Cyclin Dependent Kinase Controlling Elongation by RNA Polymerase II, Minireview for Mol. Cell. Biol. 20:2629-2634.

 

1990-1999

 

Fu, T.J., Peng, J., Price, D.H., Flores, O. (1999) Cyclin K functions as a Cdk9 regulatory subunit and participates in RNA polymerase II transcription.  J. Biol. Chem. Communication 274:34527-30.

 

Hara, R., Selby, C.P., Liu, M., Price, D.H., and Sancar, A. (1999) Human transcription release factor 2 dissociates RNA polymerases I and II stalled at a cyclobutane thymine dimer. J. Biol. Chem. 274: 24779-24786.

 

Wimmer, J., Fujinaga, K., Taube, R., Cujec, T.P., Zhu, Y., Peng, J., Price, D.H., Peterlin, B.M. (1999) Interactions between Tat and TAR and HIV replication are facilitated by human cyclin T1 but not cyclins T2a or T2b.  Virology 255:182-189.

 

Ye, M., Duus, K.M., Peng, J., Price, D.H., and Grose, C. (1999) Varicella-zoster virus Fc receptor component gI is phosphorylated on its endodomain by a cyclin dependent kinase.  J. Virol. 73:1320-1330.

 

Garriga, J., Peng, J., Parreno, M., Price, D.H., Henderson, E.E., and Grana, X. (1998) Upregulation of cyclin T1/CDK9 complexes during T cell activation.  Oncogene 17:3093-3102.

 

Liu, M., Xie, Z., and Price D.H. (1998) A human RNA polymerase II transcription termination factor is a SWI2/SNF2 family member.  J. Biol. Chem. Communication 273:25541-25544. 

 

Fujinaga, K., Cujec, T.P., Peng, J., Garraga, J., Price, D.H., Grana, X., Peterlin, B.M. (1998) The ability of P-TEFb to transactivate HIV transcription depends upon a functional kinase domain, cyclin T1, and Tat.  J. Virol. 72:7154-7159.

 

J. M. Peng, Marshall, N., and Price, D.H. (1998) Identification of a cyclin subunit required for function of Drosophila P-TEFb.  J. Biol. Chem. 273:13855-13860.

 

Peng, J., Zhu, Y., Milton, J.T., and Price, D.H. (1998) Identification of multiple cyclin subunits of human P-TEFb. Genes & Dev. 12:755-762.

 

Xie, Z. and Price D.H. (1998) Unusual nucleic acid binding properties of factor 2, an RNA polymerase II transcript release factor.  J. Biol. Chem. 273:3771-3777. 

 

Xie, Z. and Price D.H. (1997) Drosophila factor 2, an RNA polymerase II transcript release factor, has DNA-dependent ATPase activity.  J. Biol. Chem. 272:31902-31907.

 

Mancebo, H., Lee, G., Flygare, J., Tomassini, J., Luu, P., Zhu, Y., Peng, J., Blau, C., Hazuda, D., Price, D.H., and Flores, O. (1997) P-TEFb kinase is required for HIV Tat transcriptional activation in vivo and in vitro.  Genes & Dev. 11:2633-2644.

 

Zhu, Y., Pe’ery, T., Peng, J., Ramanathan, Y., Marshall, N.F., Marshall, T.K., Amendt, B.A., Mathews, M.B., Price, D.H. (1997) Transcription elongation factor P-TEFb is required for HIV-1 Tat transactivation in vitro.  Genes & Dev. 11:2622-2632.

 

Marshall, N.F., Peng, J., Xie, Z., and Price, D.H. (1996) Control of RNA polymerase II elongation potential by a novel carboxyl-terminal domain kinase.  J. Biol. Chem. 271:27176. 

 

Xie, Z. and Price, D.H. (1996) Purification of an RNA polymerase II transcript release factor from Drosophila.  J. Biol. Chem. Communication 271:11043-11046.

 

Chen, Y., Chafin, D., Price, D.H., and Greenleaf, A.L. (1996) Drosophila RNA polymerase II mutants that affect transcription elongation.  J. Biol. Chem. 271:5993-5999.

 

Chafin, D.R., Guo, H., and Price, D.H. (1995) Action of a-amanitin during pyrophosphorolysis and elongation by RNA polymerase II.  J. Biol. Chem. 270:19114-19119.

 

Marshall, N.F. and Price, D.H. (1995) Purification of P-TEFb, a transcription factor required for the transition into productive elongation.  J. Biol. Chem. Communication 270:12335-12338.

 

Kephart, D.D., Wang, B.Q., Burton, Z.F., Price, D.H., (1994) Functional analysis of the large subunit of Drosophila factor 5 (TFIIF), a general transcription factor.  J. Biol. Chem. 269:13536-13543.

 

Guo, H. and Price, D.H. (1993) Mechanism of DmS-II mediated pause suppression by Drosophila RNA polymerase II.  J. Biol. Chem. 268:18762-18770.

 

Kephart, D.D., Price, M.P., Burton, Z.F., Finkelstein, A., Greenblatt, J., and Price, D.H. (1993). Cloning of a Drosophila cDNA with sequence similarity to human transcription factor RAP74.  Nuc. Acids Res. 21:1319. 

 

Marshall, N.F. and Price, D.H. (1992) Control of formation of two distinct classes of RNA polymerase II elongation complexes.  Mol. Cell. Biol. 12:2078-2090.

 

Kephart, D.D., Marshall, N.F., and Price, D.H. (1992) Stability of Drosophila RNA polymerase II elongation complexes in vitro.  Mol. Cell. Biol. 12:2067-2077.

 

Chafin, D.R., Claussen, T.J., and Price, D.H. (1991) Identification and  purification of a yeast protein which effects elongation by RNA polymerase II.  J. Biol. Chem. 266:9256-9262.

 

Marshall, T.K., Guo, H., and Price, D.H. (1990) Drosophila RNA polymerase II elongation factor DmS-II has homology to Mouse S-II and sequence similarity to yeast PPR2.  Nuc. Acids Res. 18:6293-6298.

 

BEFORE 1990

 

Sluder, A.E., Greenleaf, A.L., and Price, D.H. (1989) Properties of a Drosophila RNA polymerase II elongation factor.  J. Biol. Chem. 264:8963-8969.

 

Price, D.H., Sluder, A.E., and Greenleaf, A.L. (1989) Dynamic interaction between a Drosophila transcription factor and RNA polymerase II.  Mol. Cell. Biol. 9:1465-1475.

 

Sluder, A.E., Price, D.H., and Greenleaf, A.L. (1988) Elongation by Drosophila RNA polymerase II: Transcription of 3’ extended DNA templates.  J. Biol. Chem. 263:9917-9925.

 

Sluder, A.E., Price, D.H., and Greenleaf, A.L. (1987) An activity necessary for in vitro transcription is a DNAse inhibitor.  Biochemie 69:1199-1205.

 

Price, D.H., Sluder, A.E., and Greenleaf, A.L. (1987) Fractionation of transcription factors for RNA polymerase II from Drosophila Kc cell nuclear extracts.  J. Biol. Chem. 262:3244-3255.

 

Morris, G.F., Price, D.H., and Marzluff, W.F. (1986) Synthesis of U1 RNA in a DNA-dependent system from sea urchin embryos.  Proc. Natl. Acad. Sci. USA 83:3674-3678.

 

Price, D.H. and Parker, C.S. (1984) The 3’ end of Drosophila histone H3 mRNA is produced by a processing activity in vitro.  Cell 38:423-429.

 

Anderson, D.M., Richter, J.D., Chamberlin, M.E., Price, D.H., Britten, R.J., Smith, D. and Davidson, E.H. (1982) Sequence organization of the poly (A) RNA synthesized and accumulated in lampbrush chromosome state Xenopus laevius oocytes.  J. Mol. Biol. 155:281-309.